An update on prime editing: recent advances and applications

Cell & Gene Therapy Insights 2023; 9(9), –0

DOI: 10.18609/cgti.2023.174

Published: 31 January
Expert Insight
Daniela Bischof

CRISPR PE is a substantial advancement of earlier CRISPR technologies, enabling the RNA-encoded installation of all substitution edits, small insertions, and small deletions without requiring neither DNA double-strand breaks nor DNA donor templates. The development of new PE technologies occurs rapidly, and recent work enhanced the PE platform by improving informatic prediction of PE outcomes, developing PEs with new capabilities, optimizing PE delivery, and introducing new PE off-target detection methods. PE is a groundbreaking technology that will continue to evolve in the coming years regarding technological capability and its deployment to the clinic. Here, we summarize the most recent progress involving PE technology and provide a perspective on its future development.