Traditional cuvette-based UV solutions with fixed pathlengths, such as using a 1cm cuvette, fail to accurately measure and often result in prolonged investigation. Dilution factors and fixed pathlength measurements are unreliable for determining the concentration of a UV chromophore in solution, lacking transferability and robustness for company-wide implementation.
The CTech™ SoloVPE® spectrophotometer features variable pathlength technology, allowing for automatic adjustment of the optical pathlength from 5µm to 15mm in 5µm increments. This technology allows for determining the appropriate pathlength and achieving linearity even with significantly higher sample concentrations without requiring dilution. The absorbance values obtained are subjected to linear slope regression analysis, with a minimum R2 value of >0.999. Variable Pathlength Technology offers speed, repeatability, and accuracy, making it suitable for global implementation to reduce turnaround time and eliminate the need for multiple personnel waiting for data results before proceeding with the next processing steps.
As Aldevron aims to enhance its analytical testing capabilities to support various product types, it is essential to qualify and validate these new technologies in compliance with regulatory guidelines. This ensures the utmost product quality and patient safety. In this webinar, Aldevron will share their strategy and the results obtained from the platform mRNA content assay, specifically focused on mRNA concentration.
What is Variable Pathlength Technology and how does is differ from traditional UV-vis fixed pathlength spectroscopy